Character: Red blood cell

illustration of red blood cell and lymphocyte nucleus with DAPI stanining

DAPI

illustration of red blood cell and lymphocyte nucleus with DAPI stanining

DAPI stands for 4′,6-diamidino-2-phenylindole and it is a fluorescent stain that binds to DNA in the nucleus. It is a stain used very often in fluorescence microscopy, when excited with ultraviolet light DAPI emits a blue light. This makes the stain very popular in fluorescence microscopy making the nuclei visible in a blue colour. As …

DAPI Read More »

red blood cell lysis illustration, red cell lysing after adding buffer

Red Blood Cell Lysis

red blood cell lysis illustration, red cell lysing after adding buffer

To analyze cells in the blood sometimes the red blood cells (RBCs) need to be removed. This can be done by lysing the cells. There are various RBC lysis buffers available that do just that. Protocols: Red Blood Cell Lysis  Protocol by Centre of Immunology and Transplantation Red Blood Cell Lysis Using ACK Lysing Buffer …

Red Blood Cell Lysis Read More »