Flowcytometry is a technology that can be used to phenotype cells by analyzing the expression of cell surface and intracellular molecules. It rapidly analyzes single cells or particles as they flow past single or multiple lasers while suspended in a buffered salt-based solution. Each particle is analyzed for visible light scatter and one or multiple fluorescence parameters.
Here are some videos that explains the concepts of flow cytometry.
If you are already using flow cytometry here are some resources you might find useful:
- Tool to streamline antibody panel design: Fluorofinder
- Video Flow Cytometry Tutorials: Doublet Discrimination
- Video Advanced Topics in Cell Sorting
McKinnon KM. Flow Cytometry: An Overview. Curr Protoc Immunol. 2018 Feb 21;120:5.1.1-5.1.11. doi: 10.1002/cpim.40. PMID: 29512141; PMCID: PMC5939936.
Flow Cytometry Animation by Cell Signaling Technology, Inc. Youtube. https://youtu.be/B2zreF2dnWk (accessed December 29, 2022).